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產(chǎn)品詳情

  • 產(chǎn)品名稱：ATP2B2 ELISA Kit(ATPase,Ca++Transporting,PlasmaMembrane2)

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：國內(nèi)供應(yīng)1
  • 產(chǎn)品文檔：
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簡單介紹：
ATP2B2 ELISA Kit(ATPase,Ca++Transporting,PlasmaMembrane2)
詳情介紹：
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ATP2B2 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sample Type Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Sensitivity 0.057 ng/mL
Characteristics Rat ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name ATP2B2 (ATP2B2 ELISA Kit Abstract)
Gene ID 24215
UniProt P11506
Pathways Sensory Perception of Sound, Regulation of Cell Size, Ribonucleoside Biosynthetic Process
Assay Time 1 - 4.5 h
Plate Pre-coated
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Expiry Date 12 months
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