91视频 - 8MAV,美女张开大腿让男人插,天天干干天天干干干天射,日韩亚洲免费在线

產(chǎn)品詳情

  • 產(chǎn)品名稱：CarbonicAnhydraseI(CA1) ELISA Kit

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：國內(nèi)供應(yīng)1
  • 產(chǎn)品文檔：
你添加了1件商品 查看購物車
簡單介紹：
CarbonicAnhydraseI(CA1) ELISA Kit
詳情介紹：
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CA1 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sample Type Biological Fluids, Cell Lysate, Plasma, Serum, Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.58 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name CA1 (CA1 ELISA Kit Abstract)
Background Synonyms: CA-I, CAI, Car1
Gene ID 759
UniProt P00915
Research Area Enzymes, Metabolism
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
Supplier Images
ELISA image for Carbonic Anhydrase I (CA1) ELISA Kit (ABIN1568739) Carbonic Anhydrase I (CA1) ELISA Kit
熟女天天干,夜夜干| 日韩成人免费性电影| 欧美做性视频在线| 人妻后入日韩精品| 屄 鸡吧 欧美日韩 国产 一区| 亚洲一区二区精品夜夜| 少妇被c操黄| 免费综合久色AⅤ视频牛牛主播 | 久久精品国产亚洲AV无码麻豆,| 日韩理论片在线观看2024| 91久久人人澡人人澡| 欧洲亚洲偷拍自拍| 欧美久久情| 欧美国产乱子精视频在线观看| 性小说免费视频| 日本美女肏屄内射| 欧美日韩久久成人在线| 亚洲精品90p| 国产无遮挡18禁网| 亚洲日韩欧美综合一区二区三| 美女自慰网站免费在线看| 欧美人妻性爱一区| 久精品官方| 逼视频五月高清无码| 性奴调教一区二区91| 国产日韩精品二三| 五月婷婷播放基地| 亚洲天天干人人爽| 日本高清 视频| 日韩人妻精品一二区| 插美女的小骚逼视频| 大香蕉在线视频看精品| 亚洲一性一乱一一区| 青青草青青青久久久| 日韩亚洲欧美影视| 舔我逼后入操我逼视频| AV综合在线免费播放| 蜜臀一区免费| 亚洲美女中文版| 欧美日韩嗯嗯啊啊| 色骚小逼|