91视频 - 8MAV,美女张开大腿让男人插,天天干干天天干干干天射,日韩亚洲免费在线

產(chǎn)品詳情

  • 產(chǎn)品名稱：Aquaporin8 ELISA Kit(AQP8)

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：國內(nèi)供應(yīng)1
  • 產(chǎn)品文檔：
你添加了1件商品 查看購物車
簡單介紹：
Aquaporin8 ELISA Kit(AQP8)
詳情介紹：
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AQP8 in human tissue homogenates, cell lysates and other biological fluids.
Sample Type Biological Fluids, Cell Lysate, Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.107 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name AQP8 (AQP8 ELISA Kit Abstract)
Background Synonyms: AQP-8,Aquaporin-8
Gene ID 343
UniProt O94778
Research Area Metabolism
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
Supplier Images
ELISA image for Aquaporin 8 (AQP8) ELISA Kit (ABIN1568485) Aquaporin 8 (AQP8) ELISA Kit
ELISA image for Aquaporin 8 (AQP8) ELISA Kit (ABIN1568485) Typical Standard Curve
91人妻在线资源| 少妇av影| 又黄又大又水视频在线观看| 欧美综合熟妇| 综合自拍av| 国产视频自拍在线| 欧美伦理久久久| 又粗又猛又长视频| 麻豆一区二区三区精品无码| 国产精品原创久久| 日韩欧美国产成人精品一区| 国产未亡人一区二区| 久久无码五| 夜夜弄夜夜爽| 欧美综合区一区二区| 成人毛片在线观看| 高清后入少妇| 午夜二区| 日韩高清无码成人电影| 欧美影院 一区| 亚洲AV五月婷婷不卡| 手机老鸦窝av在线| 亚洲一区二区三自拍| 青青草原av高清在线| 欧美一区日韩视频| 国产在线观看欧美| 欧美大黑屌xxxx| 熟女人妇交换3p视频| 97色色亚洲无码| 欧美亚洲二区图片| 国产91密视频| 嗯嗯……啊……嗯……视频| 91成人亚洲在线视频| 日韩无码久久电影麻豆传媒| 亚洲一区四季免费观看| 黄色成人小说在线| 色妹子国产综合| 亚洲精品色域| 国产熟妇丰满熟妇屁股| 国产黄色日本人妻精品一区二在线 | 久久免费福利|