91视频 - 8MAV,美女张开大腿让男人插,天天干干天天干干干天射,日韩亚洲免费在线

產(chǎn)品詳情
  • 產(chǎn)品名稱:ApoptosisInducingFactor(AIF) ELISA Kit

  • 產(chǎn)品型號(hào):
  • 產(chǎn)品廠商:國(guó)內(nèi)供應(yīng)1
  • 產(chǎn)品文檔:
你添加了1件商品 查看購(gòu)物車
簡(jiǎn)單介紹:
ApoptosisInducingFactor(AIF) ELISA Kit
詳情介紹:
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AIF in human serum, plasma, tissue homogenates and other biological fluids.
Sample Type Biological Fluids, Plasma, Serum, Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.064 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Background Synonyms: PDCD8, Programmed Cell Death 8
Gene ID 9131
UniProt O95831
Research Area Apoptosis/Necrosis
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
Supplier Images
ELISA image for Apoptosis Inducing Factor (AIF)  ELISA Kit (ABIN1028145) Apoptosis Inducing Factor (AIF) ELISA Kit
又长又粗又大三机片| 不卡a片一区二区三区蜜桃| 亚洲成人综合色区文化在线| 七七久久综合网一区二区| 日本一本道一区二区三区无码高清| 亚洲激情av一区二区| 未亡人熟女AV| 偷拍自人福利丝袜| 9999精品视频| 日本伦久久| 欧美中文字幕一区二区网站| 久久无码专区免费看| 少妇少妻精品一区二区三区 | 欧美国产日韩人妖同性| 日韩一区成人网| 亚洲视频 中文字幕| 欧美操大骚逼。| 中文少妇精品久久久久久| 久久亚洲无码电影片| 日本道人人爽| 图片区二区亚洲区| 晚上免费看B视频| 很色综合| 日本黄色私人影院| 美女色香蕉| 缅甸美女高潮内射| 午夜久久无码成人免费AV麻豆婷| 欧美色欲啪啪| AV福利中字在线看| 在线影院一区| 天天操夜夜操电影| 青青草成人综合网| 91丨九色丨海角社区| 伦理一二级视频| 久精品三级| 亚洲一区二区成人色欲| 久久精品视频天天cao| 国产一二区日韩| 久久爽无码人妻AⅤ精品噜噜噜| 精彩视频一区二区| 久久在线成人视频|