91视频 - 8MAV,美女张开大腿让男人插,天天干干天天干干干天射,日韩亚洲免费在线

產品詳情
  • 產品名稱:HumanApoptosisSignalingArrayC1

  • 產品型號:
  • 產品廠商:RayBiotech
  • 產品文檔:
你添加了1件商品 查看購物車
簡單介紹:
HumanApoptosisSignalingArrayC1
詳情介紹:
Purpose C-Series Human Apoptosis Antibody Array 1 Kit. Detects 19 phosphorylated or cleaved human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Sample Type Plasma, Cell Culture Supernatant, Serum, Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Chemiluminescent
Specificity Akt (P-Ser473) (PKB (P-Ser473)), ATM (P-Ser1981), BAD (P-Ser112), Caspase-3 (Cleaved Asp175), Caspase-7 (Cleaved Asp198), CHK1 (P-Ser296), CHK2 (P-Thr68), eIF-2a (P-Ser52), ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187), HSP27 (P-Ser82), IKBa (P-Ser32), JNK (P-Thr183/Tyr185), NFKBP65 (P-Ser536), PARP1 (Cleaved Asp214/Gly215), p27 (P-Thr198), P53 (P-Ser15), SMAD2 (Ser245/250/255), TAK1 (P-Ser412)
Characteristics
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/mL)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Components Antibody Array Membranes
Biotinylated Detection Antibody Cocktail
Blocking Buffer
Wash Buffers 1 and 2
Cell & Tissue Lysis Buffer
Detection Buffers C and D
Plastic Incubation Tray
Protease Inhibitor Cocktail (in select kits)
Material not included Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It? or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Application Notes Perform ALL incubation and wash steps under gentle rotation or rocking motion (~0.5 to 1 cycle/sec) using an orbital shaker or oscillating rocker to ensure complete and even reagent/sample coverage. Rocking/rotating too vigorously may cause foaming or bubbles to appear on the membrane surface which, should be avoided. All washes and incubations should be performed in the Incubation Tray (ITEM 10) provided in the kit. Cover the Incubation Tray with the lid provided during all incubation steps to avoid evaporation and outside debris contamination. Ensure the membranes are completely covered with sufficient sample or reagent volume during each incubation. Avoid forceful pipetting directly onto the membrane, instead, gently pipette samples and reagents into a corner of each well. Aspirate samples and reagents completely after each step by suctioning off excess liquid with a pipette. Tilting the tray so the liquid moves to a corner and then pipetting is an effective method. Optional overnight incubations may be performed for the following step to increase overall spot signal intensities:
- Sample Incubation
- Biotinylated Antibody Cocktail Incubation
- HRP-Streptavidin Incubation
Comment

The C-Series arrays feature chemiluminescent signal detection. The antibodies are spotted on nitrocellulose membrane solid supports and are handled in a very similar manner to Western blots.
All C-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.

Sample Volume 1 mL
Plate Membrane
Protocol
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Restrictions For Research Use only
Handling Advice The antibody printed side of each membrane is marked by a dash (-) or number (#) in the upper left corner. Do not allow membranes to dry out during the experiment or they may become fragile and break OR high and/or uneven background may occur. Grasp membranes by the corners or edges only using forceps. DO NOT touch printed antibody spots.
Storage -20 °C
Storage Comment For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
国产欧美视频在线观看视频| 蜜臀99久久精品久久久久久软件| 国产成人日韩在线| 亚洲精品美女一二三区| 麻豆影视国产一区二区| Julia无码无修正流出| 亚洲激情三级| 极度色诱视频国产久久久久| 中文熟女五十三级| 久久久成人午夜激情电影| 少妇精品人妻精品AV| 漂亮少妇内射一区| 免费成人福利91| 亚洲AV无码专区在线| 成人少妇二区| 激情视频偷拍| 色综合精品一区二区三四区| 欧美亚洲日韩一区二区在线| 麻豆高清无码一区二| 国产永久视频免费| 插菊花综合网1狠狠爱| 日本按摩高潮超级A中文| 熟女二区视频在线| 亚洲 综合 1区| 少妇高潮在线视频| 亚洲成人亚洲网站在线观看| 一级欧美一级欧美| 色色综合AV,就去日| 一区二区三区无码人妻1080P| 国产 欧美一区二区三区自慰| 中文视频亚洲一| 日韩片| 怼贱屄AV白浆| 午夜黄色理论| 亚洲国产中文二区二区| www.558yy com| 欧美在线激情一区二区三区 | 大香蕉三级| 无码精品另类视频在线观看| 午夜性色精品| 日韩HDav|