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  • 產品名稱:MODifiedHistonePeptideArray

  • 產品型號:
  • 產品廠商:ActiveMotif
  • 產品文檔:
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MODifiedHistonePeptideArray
詳情介紹:
Purpose The MODified Histone Peptide Array is a valuable research tool that can be used to screen antibodies, enzymes and proteins for cross-reactivity or binding interactions with histones and their post-translational modifications (PTM).
Brand MODified?
Specificity MODified Histone Peptide Array Specifications:
- Standard microscope slides (26x76 mm, white coating)
- 768 spots per slide (384 peptide-conjugate spots printed in duplicate)
- Spot-to-spot distance 1.2 mm
- Peptides are covalently bound to cellulose via C-terminus
- Arrays contain control peptides and location marks
Characteristics The MODified Histone Peptide Array is a valuable research tool that can be used to screen antibodies, enzymes and proteins for cross-reactivity or binding interactions with histones and their post-translational modifications (PTM). The MODified Histone Peptide Arrays screen 59 acetylation, methylation, phosphorylation, and citrullination modifications on the N-terminal tails of histones H2A, H2B, H3 and H4. Each peptide array contains 384 unique histone modification combinations in duplicate, including up to four separate modifications on the same 19mer peptide, offering the most extensive coverage available for commercial arrays of similar format. This extensive coverage of histone modifications enables the study of not only individual sites, but also the effects of neighboring modifications on recognition and binding. The unique synthesis and conjugation methods ensure greater than 95% purity of bound peptides and high peptide density at each spot, which is advantageous for analysis of interaction sites with low binding constants. The simple array protocol works like a Western blot; no special equipment needed.
Components MODified Histone Peptide Array - peptide arrays contain 384 unique histone modification combinations. Arrays are available individually or in packs of 5 arrays.
Application Notes The MODified Histone Peptide Arrays can be used to screen antibodies, enzymes and proteins for cross reactivity or binding interactions with histones and their post-translational modifications. Following a blocking step, the array is incubated with either antibodies, proteins or enzymes for one hour, washed and then incubated for one hour with either a horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibody. Developing solution is then added and the image is captured using film or a CCD camera; no special equipment is needed. The MODified Histone Peptide Arrays can also be analyzed using the LI-COR??Odyssey??infrared imaging system and infrared detection reagents
Comment

MODified Histone Peptide Array - peptide arrays contain 384 unique histone modification combinations.
The MODified Array Labeling Kit (ABIN2866137) can be used to study antibody and enzyme interactions.
The MODified Protein Domain Binding Kit (ABIN 2866149) is designed to interrogate the binding specificity of His-tagged protein reading domains.

Restrictions For Research Use only
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