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產(chǎn)品詳情

  • 產(chǎn)品名稱：Jurkatnuclearextract(TPA+CIstimulated)

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：ActiveMotif
  • 產(chǎn)品文檔：
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簡單介紹：
Jurkatnuclearextract(TPA+CIstimulated)
詳情介紹：
Characteristics

Origin: Human T lymphoblast, acute T cell leukemia

Components 2 x 100 μg of Jurkat nuclear extract (TPA + CI stimulated) at 2.5 μg/μl
Restrictions For Research Use only
Concentration 2.5 μg/μL
Buffer Nuclear extracts are supplied in dilution buffer (20?mM Hepes (?pH 7.9), 100 mM KCl, 1?mM MgCl2, 20?% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured in medium supplemented with 50?ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 μM calcium ionophore A23187 (CI) for two hours at 37?°C immediately prior to harvesting.
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
Storage -80 °C
Storage Comment To ensure stability, extracts should be stored at -80°C.
Supplier Images
Activity Assay (AcA) image for Jurkat nuclear extract (TPA + CI stimulated) (ABIN2669798) Jurkat nuclear extract (TPA + CI stimulated)
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