91视频 - 8MAV,美女张开大腿让男人插,天天干干天天干干干天射,日韩亚洲免费在线

產(chǎn)品詳情
  • 產(chǎn)品名稱:AngiotensinII ELISA Kit(AngII)

  • 產(chǎn)品型號(hào):
  • 產(chǎn)品廠商:國(guó)內(nèi)供應(yīng)1
  • 產(chǎn)品文檔:
你添加了1件商品 查看購(gòu)物車
簡(jiǎn)單介紹:
AngiotensinII ELISA Kit(AngII)
詳情介紹:
Purpose The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AngII in rabbit serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sample Type Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 9.66 pg/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name AngII (Ang II ELISA Kit Abstract)
Pathways JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.

Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
亚洲欧美日本区| 国产在线日韩欧美另类一区| 亚洲精品27P| 欧美日韩一区二区三区色综合| 午夜精品小视频在线| 亚洲日韩昭和在线| 久久中文字幕综合网| 一本道亚洲综合成人| 越南骚妇免费在线| 大香蕉3月婷婷| 熟女少妇成人免费在线| 亚洲伊人五月天久久久| 国产日韩午夜精品欧美视线| 大大香蕉伊人| 日欧中文字幕| 欧美香蕉在| 国产麻豆精选一区| 日韩 中文字幕 有码| 美女搞鸡巴视频在线看| 亚州欧美熟女| AAA欧美日韩AAA| 亚洲AV综合H交| 日本三级久久久久久中老年人| 亚洲天堂精品流出| 亚洲黑丝无码在线| 亚洲自拍偷拍在线点播| 內射少妇人妻 在线播放| 欧美色人妖百度| 国产区一区无码| 欧美骚女插逼网| 日韩天天操操| 欧美日韩一区人妻在线播放| 色呦呦国产精品漫画| 插插影院一区欧美| 日本一道本欧美激情| 大香蕉12i| 久久国产V综合V亚洲欧美蜜臀| 日本一区二区三区九九| 一区二区三区欧美日本| 武清区| 久久毛片精品网|