91视频 - 8MAV,美女张开大腿让男人插,天天干干天天干干干天射,日韩亚洲免费在线

產(chǎn)品詳情

  • 產(chǎn)品名稱：MMP2 ELISA Kit(MatrixMetalloproteinase2)

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：國內(nèi)供應(yīng)1
  • 產(chǎn)品文檔：
你添加了1件商品 查看購物車
簡單介紹：
MMP2 ELISA Kit(MatrixMetalloproteinase2)
詳情介紹：
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MMP2 in mouse serum, plasma, tissue homogenates and other biological fluids.
Sample Type Biological Fluids, Plasma, Serum, Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.065 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name MMP2 (MMP2 ELISA Kit Abstract)
Background Synonyms: CLG4, CLG4A, MMP-II, MONA, TBE-1, Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase
Gene ID 17390
UniProt P33434
Research Area Extracellular Matrix
Pathways Activation of Innate immune Response
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
Supplier Images
ELISA image for Matrix Metalloproteinase 2 (MMP2) ELISA Kit (ABIN1029244) Matrix Metalloproteinase 2 (MMP2) ELISA Kit
啊啊啊啊啊嗯嗯嗯额在线| 人妻交换诱惑中出| 国产日韩在线三级| 日本不卡偷拍| 亚洲午夜看电影| 亚洲午夜理论| 久久亚洲中文| 九九亚洲三级片| 天天夜夜人人精品网| 欧美日韩国产在线一区二区三区四区| 91视频成人版亚洲| 女女同国产精品| 人人操人人捏| 欧美日韩三七七色噜噜| 婷婷国产精品麻豆| 裸毛一区二区| 亚洲v在线播放| 成人性爱免费国产麻豆视频| 久久精品.com| 日逼免费视频规看| 性爱高潮喷水抽搐视频| 久久人妻黄色片| 国产欧美婷婷色| 日韩久久成人| 小吊妞免费视频网址| 久亚洲三级| 曰本不卡视频| 日韩欧美 h| 国产老熟女3| 九九九九九九香蕉| 日韩一区,国产二区| 日本久久一次黄色| 日韩午夜福利一区二区福利| 亚欧精品一区二区二区| 色欲之一区二区| 午夜寂寞少妇免费视| 天天操天天碰| 经典福利视频| 亚洲国产欧美日韩成人综合,日韩| 国产黄色美女免费网| 综合一区色|