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Buffer
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The HeLa whole-cell extract has been collected in Lysis Buffer after a 30-minute mock stimulation. Lysis Buffer consists of 20 mM Hepes?pH 7.5, 350 mM NaCl, 20?% glycerol, 1?% Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA. The protein content has been determined by a Bradford-based assay.
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